Lab Protocols:

• Isotonic kinetoplast isolation (L. tarentolae) (Nitrogen decavitation breakage)
• Hypotonic kinetoplast isolation (L. tarentolae)
• Isotonic kinetoplast isolation (L. tarentolae) (Stansted breakage)
• Hypotonic kinetoplast isolation (T. brucei)
• Searching for gRNAs knowing the edited mRNA sequence
• Laragen sequencing protocol
• Diagnostic PCR of clones
• T7 transcription
• Agar plating of Leishmania
• RNA isolation for microarrays (S. Beverley)
• In gel trypsin digestion - for mass spectrometry (Falik lab, UC Berkeley)
• Native gels for large complexes.
• Modified TAP protocol
• Modified SAP-TAP protocol
• Agarose formaldehyde gel
• Northern hybridization
• Giemsa staining of cells
• Oligo purification and primer extension analysis.
• Plasmid mini-prep procedure. (AMS method)
• RACE procedure.
• Long PCR
• Chromosome gels
• Preparation of protein for micro-sequencing N-terminus.
• Southern blotting.
• Transformation of Trypanosoma brucei.
• Total cell RNA isolation.
• Sequencing (Sequenase)
• Blue Native Gels
• Modified procedure for diagnosis of T. cruzi in blood by PCR of minicircle DNA.
• Adenylation of putative RNA ligase proteins in L. tarentolae mitochondria.
• Glycerol gradient sedimentation of mitochondrial extract
• RT PCR
• Large scale plasmid DNA isolation procedure
• Buffer design web site
• Metric conversion web site
• Sequence shuffle site
• Wu Blast server (EMBL)
• Technique Chapter (PDF file: Click here to download Adobe Acrobat Reader)
• Another Technique Chapter (PDF file: Click here to download Adobe Acrobat Reader)
• Life Technology protocols
• Donelson lab protocols
• Some Basic Conversions and Calculations
• Conversion DNA picomoles to micrograms
• Calculation of standard deviation