Lab Protocols:
Isotonic kinetoplast
isolation
(L. tarentolae) (Nitrogen decavitation breakage)
Hypotonic kinetoplast
isolation
(L. tarentolae)
Isotonic kinetoplast
isolation
(L. tarentolae) (Stansted breakage)
Hypotonic kinetoplast
isolation
(T. brucei)
Searching for gRNAs
knowing the edited mRNA sequence
Laragen sequencing protocol
Diagnostic PCR of clones
T7 transcription
Agar plating of Leishmania
RNA isolation for microarrays (S. Beverley)
In gel trypsin digestion
- for mass spectrometry (Falik lab, UC Berkeley)
Native gels
for large complexes.
Modified TAP protocol
Modified
SAP-TAP protocol
Agarose formaldehyde
gel
Northern hybridization
Giemsa
staining
of cells
Oligo
purification and primer extension analysis.
Plasmid mini-prep
procedure
. (AMS
method
)
RACE
procedure.
Long
PCR
Chromosome
gels
Preparation of
protein
for micro-sequencing N-terminus.
Southern
blotting
.
Transformation
of Trypanosoma brucei.
Total cell RNA
isolation.
Sequencing
(Sequenase)
Blue Native
Gels
Modified
procedure
for diagnosis of
T. cruzi
in blood by PCR of minicircle DNA.
Adenylation
of putative RNA ligase proteins in
L. tarentolae
mitochondria.
Glycerol gradient
sedimentation of mitochondrial extract
RT PCR
Large scale plasmid DNA isolation procedure
Buffer design
web site
Metric conversion
web site
Sequence shuffle site
Wu Blast server
(EMBL)
Technique
Chapter
(PDF file: Click
here
to download Adobe Acrobat Reader)
Another Technique
Chapter
(PDF file: Click
here
to download Adobe Acrobat Reader)
Life Technology protocols
Donelson lab protocols
Some Basic Conversions and Calculations
Conversion DNA picomoles to micrograms
Calculation of standard deviation
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