LOCUS pEKA2-revised 8355 bp DNA circular
FEATURES Location/Qualifiers
rep_origin complement(7924..8300)
/vntifkey="33"
/label=ORI
/note="E. coli pMB2 replication origin"
CDS 2..785
/vntifkey="4"
/label=LtHGPRT\ORF\+\3'\UTR
splicing_signal 786..956
/vntifkey="38"
/label=Aldolase\5'\UTR\SAS
splicing_signal 962..1047
/vntifkey="38"
/label=PARP\SAS
/note="Accession S60066. There is SAS redundancy due to need for site during cloning."
3'UTR 3941..4095
/vntifkey="50"
/label=3'\aldolase\UTR
/note="Accession X52586"
CDS 4221..5261
/vntifkey="4"
/label=HygroR
/note="Accession K01193"
3'UTR 5277..5554
/vntifkey="50"
/label=Actin\3'\UTR
/note="Accession M20310"
CDS 6500..7360
/vntifkey="4"
/label=AmpR
CDS 1192..3876
/vntifkey="4"
/label=T7\RNA\polymerase
BASE COUNT 2013 a 2122 c 2213 g 2007 t
ORIGIN
1 ctgatgagca actcagtcaa gtccccttcc gaccatgtcg gtgatgtggg gcggcgcaac
61 tacccgatgt cggcccgcac gctcgtcaca caggagcagg tgtgggccgc cacagcgaag
121 tgcgcaaaga agattgcagc ggactacaag gacttccact taacggccga caacccgctc
181 tacttgctgt gcgtgctcaa gggcagcttc atcttcacgg ccgacctcgc ccgctttctg
241 gccgacgagg gggtgcctgt gaaggtggag tttatctgcg ctagctcgta cggctcgggc
301 gtggagacgt cgggtcaggt gcgcatgctt ctcgacgtgc gcgactccgt ggaaaaccgc
361 catattatgc ttgtcgagga catagtcgac agcgccatca cactgcagta cctgatgcgg
421 ttcatgctcg ccaagaagcc cgcctcgctg aagaccgtag tacttctgga caagccgtcg
481 gggcgcaagg tggatgtgct ggttgactac cccgtcatca cgatcccaca cgcgtttgtg
541 atcggctacg tatggacctt cgccgatcta cggggagtgc ctgatatctg cgttctaaag
601 aaggaatact atgagaaggc ggagagcaag ctgtagtggc gacgcgttac cactcgtgct
661 ggcgggcaag tgcgccgctc tctttcctcg tgaatgccct tttcacggac ccccccacac
721 acacgtacag ggacatgcta acaagcatgc acgtggccgg tgaaagtgac gaggctgcag
781 tgacgcgcgt ttccttacat atttctctaa caggcacgga agcctaacaa atacacttgg
841 cttatttttt tgccccctca tgtcttgtac aaatatttgc gatagcttag ctatcagcca
901 cattaatcaa acaagtatac caacaagccc gaaaacataa actcaactgc aacgaagctg
961 ggctgcacgc gccttcgagt tttttttcct tttccccatt tttttcaact tgaagacttc
1021 aattacacca aaaagtaaaa ttcacaagct gatctgatcc ggatttacta actggaagag
1081 gcactaaatg aacacgatta acatcgctaa gaacgaattc ctcgagcctc caaaaaagaa
1141 gagaaaggtc gaattctctg atccggattt actaactgga agaggcacta aatgaacacg
1201 attaacatcg ctaagaacga attcctcgag cctccaaaaa agaagagaaa ggtcgaattc
1261 tctgacatcg aactggctgc tatcccgttc aacactctgg ctgaccatta cggtgagcgt
1321 ttagctcgcg aacagttggc ccttgagcat gagtcttacg agatgggtga agcacgcttc
1381 cgcaagatgt ttgagcgtca acttaaagct ggtgaggttg cggataacgc tgccgccaag
1441 cctctcatca ctaccctact ccctaagatg attgcacgca tcaacgactg gtttgaggaa
1501 gtgaaagcta agcgcggcaa gcgcccgaca gccttccagt tcctgcaaga aatcaagccg
1561 gaagccgtag cgtacatcac cattaagacc actctggctt gcctaaccag tgctgacaat
1621 acaaccgttc aggctgtagc aagcgcaatc ggtcgggcca ttgaggacga ggctcgcttc
1681 ggtcgtatcc gtgaccttga agctaagcac ttcaagaaaa acgttgagga acaactcaac
1741 aagcgcgtag ggcacgtcta caagaaagca tttatgcaag ttgtcgaggc tgacatgctc
1801 tctaagggtc tactcggtgg cgaggcgtgg tcttcgtggc ataaggaaga ctctattcat
1861 gtaggagtac gctgcatcga gatgctcatt gagtcaaccg gaatggttag cttacaccgc
1921 caaaatgctg gcgtagtagg tcaagactct gagactatcg aactcgcacc tgaatacgct
1981 gaggctatcg caacccgtgc aggtgcgctg gctggcatct ctccgatgtt ccaaccttgc
2041 gtagttcctc ctaagccgtg gactggcatt actggtggtg gctattgggc taacggtcgt
2101 cgtcctctgg cgctggtgcg tactcacagt aagaaagcac tgatgcgcta cgaagacgtt
2161 tacatgcctg aggtgtacaa agcgattaac attgcgcaaa acaccgcatg gaaaatcaac
2221 aagaaagtcc tagcggtcgc caacgtaatc accaagtgga agcattgtcc ggtcgaggac
2281 atccctgcga ttgagcgtga agaactcccg atgaaaccgg aagacatcga catgaatcct
2341 gaggctctca ccgcgtggaa acgtgctgcc gctgctgtgt accgcaagga cagggctcgc
2401 aagtctcgcc gtatcagcct tgagttcatg cttgagcaag ccaataagtt tgctaaccat
2461 aaggccatct ggttccctta caacatggac tggcgcggtc gtgtttacgc cgtgtcaatg
2521 ttcaacccgc aaggtaacga tatgaccaaa ggactgctta cgctggcgaa aggtaaacca
2581 atcggtaagg aaggttacta ctggctgaaa atccacggtg caaactgtgc gggtgtcgat
2641 aaggttccgt tccctgagcg catcaagttc attgaggaaa accacgagaa catcatggct
2701 tgcgctaagt ctccactgga gaacacttgg tgggctgagc aagattctcc gttctgcttc
2761 cttgcgttct gctttgagta cgctggggta cagcaccacg gcctgagcta taactgctcc
2821 cttccgctgg cgtttgacgg gtcttgctct ggcatccagc acttctccgc gatgctccga
2881 gatgaggtag gtggtcgcgc ggttaacttg cttcctagtg agaccgttca ggacatctac
2941 gggattgttg ctaagaaagt caacgagatt ctacaagcag acgcaatcaa tgggaccgat
3001 aacgaagtag ttaccgtgac cgatgagaac actggtgaaa tctctgagaa agtcaagctg
3061 ggcactaagg cactggctgg tcaatggctg gctcacggtg ttactcgcag tgtgactaag
3121 cgttcagtca tgacgctggc ttacgggtcc aaagagttcg gcttccgtca acaagtgctg
3181 gaagatacca ttcagccagc tattgattcc ggcaagggtc cgatgttcac tcagccgaat
3241 caggctgctg gatacatggc taagctgatt tgggaatctg tgagcgtgac ggtggtagct
3301 gcggttgaag caatgaactg gcttaagtct gctgctaagc tgctggctgc tgaggtcaaa
3361 gataagaaga ctggagagat tcttcgcaag cgttgcgctg tgcattgggt aactcctgat
3421 ggtttccctg tgtggcagga atacaagaag cctattcaga cgcgcttgaa cctgatgttc
3481 ctcggtcagt tccgcttaca gcctaccatt aacaccaaca aagatagcga gattgatgca
3541 cacaaacagg agtctggtat cgctcctaac tttgtacaca gccaagacgg tagccacctt
3601 cgtaagactg tagtgtgggc acacgagaag tacggaatcg aatcttttgc actgattcac
3661 gactccttcg gtaccattcc ggctgacgct gcgaacctgt tcaaagcagt gcgcgaaact
3721 atggttgaca catatgagtc ttgtgatgta ctggctgatt tctacgacca gttcgctgac
3781 cagttgcacg agtctcaatt ggacaaaatg ccagcacttc cggctaaagg taacttgaac
3841 ctccgtgaca tcttagagtc ggacttcgcg ttcgcgtaac gccaaatcaa tacgactcac
3901 tatagaggga caaactcaag gtcattcgca agagtggccg gatcctgccc atttagttag
3961 ttggcttttc ccttgtctcg tgtcttttcc gtggaaaggt tcccggagta atctgatggc
4021 acagcaggga ggtgcgcctg caggttggtt aggaaggggg gatgatgtaa aagaagaaaa
4081 tggggggatt cgagcccggg cacagcaagg tcttctgaaa ttcatgtttt tttttttttt
4141 actctgcatt gcagtctccg ctcttattta gttttgcttt acgtaaggtc tcgttgctgc
4201 cataaaataa gctactagtg atgaaaaagc ctgaactcac cgcgacgtct gtcgagaagt
4261 ttctgatcga aaagttcgac agcgtctccg acctgatgca gctctcggag ggcgaagaat
4321 ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt cctgcgggta aatagctgcg
4381 ccgatggttt ctacaaagat cgttatgttt atcggcactt tgcatcggcc gcgctcccga
4441 ttccggaagt gcttgacatt ggggaattca gcgagagcct gacctattgc atctcccgcc
4501 gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga actgcccgct gttctgcagc
4561 cggtcgcgga ggccatggat gcgatcgctg cggccgatct tagccagacg agcgggttcg
4621 gcccattcgg accgcaagga atcggtcaat acactacatg gcgtgatttc atatgcgcga
4681 ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg
4741 tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga ctgccccgaa gtccggcacc
4801 tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga caatggccgc ataacagcgg
4861 tcattgactg gagcgaggcg atgttcgggg attcccaata cgaggtcgcc aacatcttct
4921 tctggaggcc gtggttggct tgtatggagc agcagacgcg ctacttcgag cggaggcatc
4981 cggagcttgc aggatcgccg cggctccggg cgtatatgct ccgcattggt cttgaccaac
5041 tctatcagag cttggttgac ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg
5101 acgcaatcgt ccgatccgga gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg
5161 cggccgtctg gaccgatggc tgtgtagaag tactcgccga tagtggaaac cgacgcccca
5221 gcactcgtcc gagggcaaag gaatagagta gatgccgacc gaacaaggat cgatcctaac
5281 accgggttgt gtggccaaaa ttgttctgta gtcgctgtga gttgacacgg ctagtgctta
5341 tgattttcct cgcgtgtggt gcctgtactc agccctatgc cttatttgca acacatttac
5401 gtacagcgca caagagaaga gaagatcact tgaagataat aaatataggg ttgtaggcat
5461 cttgtttaac tcaaattttc tcgtcttggt gtgtcgacat gattgaaata gtgccaccag
5521 ttgtgtttga tgcgtttgtt atctatgcag tattctgcaa ggccttgcag gcatgcaagc
5581 tagcttgtat tctatagtgt cacctaaatc gtatgtgtat gatacataag gttatgtatt
5641 aattgtagcc gcgttctaac gacaatatgt acaagcctaa ttgtgtagca tctggcttac
5701 tgaagcagac cctatcatct ctctcgtaaa ctgccgtcag agtcggtttg gttggacgaa
5761 ccttctgagt ttctggtaac gccgttccgc accccggaaa tggtcagcga accaatcagc
5821 agggtcatcg ctagccagat cctctacgcc ggacgcatcg tggccggcat caccggcgcc
5881 acaggtgcgg ttgctggcgc ctatatcgcc gacatcaccg atggggaaga tcgggctcgc
5941 cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg
6001 ggactgttgg gcgccatctc cttgcaccat tccttgcggc ggcggtgctc aacggcctca
6061 acctactact gggctgcttc ctaatgcagg agtcgcataa gggagagcgt cgatatggtg
6121 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac
6181 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
6241 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
6301 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
6361 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
6421 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
6481 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
6541 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
6601 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
6661 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
6721 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
6781 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
6841 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
6901 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
6961 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
7021 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
7081 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
7141 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
7201 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
7261 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
7321 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
7381 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
7441 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
7501 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
7561 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
7621 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
7681 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
7741 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
7801 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
7861 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
7921 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
7981 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
8041 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
8101 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
8161 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
8221 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
8281 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
8341 gattcattaa tgcag
//