LOCUS pLEW100 7417 bp DNA circular 2-JUN-2000
SOURCE
ORGANISM
COMMENT CAn remove luciferase with HindIII and BamHI digestion.
COMMENT This file is created by Vector NTI
http://www.informaxinc.com/
COMMENT VNTDATE|196006586|
COMMENT VNTDBDATE|196545832|
COMMENT VNTNAME|pLEW100|
COMMENT VNTAUTHORNAME|Tristan|
COMMENT VNTAUTHOREML|simpson@hhmi.ucla.edu|
COMMENT VNTOAUTHORNAME|Sigfried|
FEATURES Location/Qualifiers
rRNA complement(8..712)
/vntifkey="58"
/label=rRNA\spacer
3'UTR 719..1003
/vntifkey="50"
/label=Actin\3'\UTR
CDS complement(1004..1476)
/vntifkey="4"
/label=Ble
5'UTR 1477..1593
/vntifkey="52"
/label=Actin\5'\SAS
promoter complement(1599..1620)
/vntifkey="30"
/label=T7\promoter
promoter 1629..1953
/vntifkey="29"
/label=Tc-PARP\promoter
/note="Tetracycline responsive PARP promoter xontaining 2 tandem tet operators"
misc_feature 1903..1921
/vntifkey="21"
/label=tet\operator
misc_feature 1882..1899
/vntifkey="21"
/label=tet\operator
splicing_signal 1954..2042
/vntifkey="38"
/label=PARP\SAS
3'UTR 3925..4630
/vntifkey="50"
/label=Aldolase\long\3'\UTR\from\pHD103
misc_feature 4631..7
/vntifkey="21"
/label=pGEM4
rep_origin 6987..7362
/vntifkey="33"
/label=E.\coli\ori
CDS 5562..6422
/vntifkey="4"
/label=AmpR
CDS 2117..3766
/vntifkey="4"
/label=luciferase
BASE COUNT 1950 a 1710 c 1859 g 1895 t 3 others
ORIGIN
1 gaattcgagc tcatatagtt ggtatgtatt ctaattccag actactggcg tggataaaca
61 tgtcccctga ttaaaaggaa agattccata gcccattagt gcaaagataa ttggtacacc
121 gtcaaaaaca catgggagac cacacgatac gacactgtga ccgtagcatc aattccgcac
181 tcgatataac tctatcgaag aacttccatg gtacaaactg gtcgccacgg tctgctccac
241 acggagatca tcgtatcatt tttatcgata gcggccgcta tcgatgtatg ccttggccct
301 gatggcatgc caatttcact acaacggact atgtggaccc cgttatcatg gaaatgcgct
361 agttggagga agttagaccg cgccggaaaa gagaggggta gagaaaatga caacttggaa
421 gatatccaca cacgcacggt gaaacgttag caacaattat tagggaagca cgcttgccct
481 agtcccacga gtaaccaaga ctccaaaagc ctttctggca cagagagcga gccgaaatgg
541 aaaagagaaa caatgcctgc actaacacta ctgagcgatt cgcctcgccg cggaggaccg
601 aatactaata acgacacttg cggtcaaaaa gtagaagaac aaatgctcaa cgatgagtga
661 atcaggttag ggtagttgga aaattataca gaatgtcttt ggcaacacac cggcnnnctt
721 gcatgcctgc aaggccttgc agaatactgc atagataaca aacgcatcaa acacaactgg
781 tggcactatt tcaatcatgt cgacacacca agacgagaaa atttgagtta aacaagatgc
841 ctacaaccct atatttatta tcttcaagtg atcttctctt ctcttgtgcg ctgtacgtaa
901 atgtgttgca aataaggcat agggctgagt acaggcacca cacgcgagga aaatcataag
961 cactagccgt gtcaactcac agcgactaca gaacaatttt ggccacacaa cccggtgtta
1021 ggatctccga ggcctgggac ccgtgggccg ccgtcggacc ggcggtgttg gtcggcgtcg
1081 gtcagtcctg ctcctcggcc acgaagtgca cgcagttgcc ggccgggtcg cgcagggcga
1141 actcccgccc ccacggctgc tcgccgatct cggtcatggc cggcccggag gcgtcccgga
1201 agttcgtgga cacgacctcc gaccactcgg cgtacagctc gtccaggccg cgcacccaca
1261 cccaggccag ggtgttgtcc ggcaccacct ggtcctggac cgcgctgatg aacagggtca
1321 cgtcgtcccg gaccacaccg gcgaagtcgt cctccacgaa gtcccgggag aacccgagcc
1381 ggtcggtcca gaactcgacc gctccggcga cgtcgcgcgc ggtgagcacc ggaacggcac
1441 tggtcaactt ggccatggtg atatagctta ttttatggca gcaacgagac cttacgtaaa
1501 gcaaaactaa ataagagcgg agactgcaat gcagagtaaa aaaaaaaaaa acatgaattt
1561 cagaagacct tgctgtgccc ccggtacggg ctagatctcc ctatagtgag tcgtattaat
1621 caggtaccgt cattggggtt aagcggaaag gtgtgtgtca gtaggttgtg aggtgaaagc
1681 gttttcagat gcatagtgag cttaatgtcc ttttcacagt atatcgtgtc tgataggtat
1741 ctcttattag tatagtcgaa tactagtcaa tagtgcgttt tgtgcaaaat gtccattttg
1801 tggcagtgat ggggttgttt tatgctattc cgtgtctctg ggtgggcgtg cattgaaaat
1861 aggggttatc gggtagggat ctccctatca gtgatagaga tctccctatc agtgatagag
1921 atccctgagt actgagttta acatgttctc gtcccgggct gcacgcgcct tcgagttttt
1981 tttccttttc cccatttttt tcaacttgaa gacttcaatt acaccaaaaa gtaaaattca
2041 caagcttgga attcctttgt gttacattct tgaatgtcgc tcgcagtgac attagcattc
2101 cggtactgtt ggtaaaatgg aagacgccaa aaacataaag aaaggcccgg cgccattcta
2161 tcctctagag gatggaaccg ctggagagca actgcataag gctatgaaga gatacgccct
2221 ggttcctgga acaattgctt ttacagatgc acatatcgag gtgaacatca cgtacgcgga
2281 atacttcgaa atgtccgttc ggttggcaga agctatgaaa cgatatgggc tgaatacaaa
2341 tcacagaatc gtcgtatgca gtgaaaactc tcttcaattc tttatgccgg tgttgggcgc
2401 gttatttatc ggagttgcag ttgcgcccgc gaacgacatt tataatgaac gtgaattgct
2461 caacagtatg aacatttcgc agcctaccgt agtgtttgtt tccaaaaagg ggttgcaaaa
2521 aattttgaac gtgcaaaaaa aattaccaat aatccagaaa attattatca tggattctaa
2581 aacggattac cagggatttc agtcgatgta cacgttcgtc acatctcatc tacctcccgg
2641 ttttaatgaa tacgattttg taccagagtc ctttgatcgt gacaaaacaa ttgcactgat
2701 aatgaattcc tctggatcta ctgggttacc taagggtgtg gcccttccgc atagaactgc
2761 ctgcgtcaga ttctcgcatg ccagagatcc tatttttggc aatcaaatca ttccggatac
2821 tgcgatttta agtgttgttc cattccatca cggttttgga atgtttacta cactcggata
2881 tttgatatgt ggatttcgag tcgtcttaat gtatagattt gaagaagagc tgtttttacg
2941 atcccttcag gattacaaaa ttcaaagtgc gttgctagta ccaaccctat tttcattctt
3001 cgccaaaagc actctgattg acaaatacga tttatctaat ttacacgaaa ttgcttctgg
3061 gggcgcacct ctttcgaaag aagtcgggga agcggttgca aaacgcttcc atcttccagg
3121 gatacgacaa ggatatgggc tcactgagac tacatcagct attctgatta cacccgaggg
3181 ggatgataaa ccgggcgcgg tcggtaaagt tgttccattt tttgaagcga aggttgtgga
3241 tctggatacc gggaaaacgc tgggcgttaa tcagagaggc gaattatgtg tcagaggacc
3301 tatgattatg tccggttatg taaacaatcc ggaagcgacc aacgccttga ttgacaagga
3361 tggatggcta cattctggag acatagctta ctgggacgaa gacgaacact tcttcatagt
3421 tgaccgcttg aagtctttaa ttaaatacaa aggatatcag gtggcccccg ctgaattgga
3481 atcgatattg ttacaacacc ccaacatctt cgacgcgggc gtggcaggtc ttcccgacga
3541 tgacgccggt gaacttcccg ccgccgttgt tgttttggag cacggaaaga cgatgacgga
3601 aaaagagatc gtggattacg tcgccagtca agtaacaacc gcgaaaaagt tgcgcggagg
3661 agttgtgttt gtggacgaag taccgaaagg tcttaccgga aaactcgacg caagaaaaat
3721 cagagagatc ctcataaagg ccaagaaggg cggaaagtcc aaattgtaaa atgtaactgt
3781 attcagcgat gacgaaattc ttagctattg taatattata tgcaaattga tgaatggtaa
3841 ttttgtaatt gtgggtcact gtactatttt aacgaataat aaaatcaggt ataggtaact
3901 aaaaaggaat tcgagctcgg atcctgccca tttagttggc ttttcccttg tctcgtgtct
3961 tttccgtgga aaggttcccg gagtaatctg atggcacagc agggaggtgc gcctgcaggt
4021 tggttaggaa ggggggatga tgtaaaagaa gaaaatgggg ggatatctta tgtttaagag
4081 gataaataat gtgaaggggc tttatatgct tgcttggttg tctcgttggt gcatggggaa
4141 tctgcatgtt tgctttggag cacgcgtggt acatcaatgg gatcatatct tgctgcctcc
4201 cgcagctcac cgtgcgagct gccgggaccc cgtttctcat aggcgtgtgc accttccgtc
4261 tgagacctgt aaatggttaa aaaggaatat ataattacct tttgaaaagt ggtaaaaaac
4321 gaatatatct ttttttttgg ttttaatacg ctcttttgtg tgtatgagag gaataaataa
4381 gtgtgtgtgt gtgtgtgtgt tgttgttgtt gaaaaaatga aggacacgaa agcgctcatt
4441 aagggtgggg atacaccagg cataattatc cgaaaatatt ttgatgcacc aaataagtga
4501 acatactgac gaaatcaaag agttggagga tggataggga ggcctcattg gcagtggtaa
4561 attgatttga cttgaatatg ataccctctt actgtttcat tgccactaca agttggtttc
4621 cttcccctgc aggcatgcaa gctagcttgt attctatagt gtcacctaaa tcgtatgtgt
4681 atgatacata aggttatgta ttaattgtag ccgcgttcta acgacaatat gtacaagcct
4741 aattgtgtag catctggctt actgaagcag accctatcat ctctctcgta aactgccgtc
4801 agagtcggtt tggttggacg aaccttctga gtttctggta acgccgttcc gcaccccgga
4861 aatggtcagc gaaccaatca gcagggtcat cgctagccag atcctctacg ccggacgcat
4921 cgtggccggc atcaccggcg ccacaggtgc ggttgctggc gcctatatcg ccgacatcac
4981 cgatggggaa gatcgggctc gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat
5041 ggtggcaggc cccgtggccg ggggactgtt gggcgccatc tccttgcacc attccttgcg
5101 gcggcggtgc tcaacggcct caacctacta ctgggctgct tcctaatgca ggagtcgcat
5161 aagggagagc gtcgatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
5221 gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
5281 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
5341 cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta
5401 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
5461 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
5521 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
5581 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
5641 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
5701 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
5761 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
5821 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
5881 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
5941 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
6001 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
6061 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
6121 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
6181 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
6241 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
6301 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
6361 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
6421 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
6481 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
6541 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
6601 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
6661 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
6721 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
6781 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
6841 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
6901 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
6961 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
7021 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
7081 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
7141 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
7201 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
7261 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
7321 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
7381 cgcctctccc cgcgcgttgg ccgattcatt aatgcag
//