LOCUS pLEW90 6190 bp DNA circular 8-JUN-2000
SOURCE
ORGANISM
COMMENT This file is created by Vector NTI
http://www.informaxinc.com/
COMMENT VNTDATE|138454045|
COMMENT VNTDBDATE|197046824|
COMMENT VNTNAME|pLEW90|
COMMENT VNTAUTHORNAME|Tristan|
COMMENT VNTAUTHOREML|simpson@hhmi.ucla.edu|
FEATURES Location/Qualifiers
rep_origin complement(5760..6135)
/vntifkey="33"
/label=E.\coli\ori
/note="E. coli pMB2 replication origin"
CDS 1..905
/vntifkey="4"
/label=B-tublulin
/note="3' region of B-tubuline gene. Accession k02836 Linearizing at NotI site will intergrate plasmid in the B-tubulin locus."
5'UTR 908..1144
/vntifkey="52"
/label=Ald\SAS
/note="Aldolase splicing acceptor site. Accessioin X52586"
misc_feature 1152..1776
/vntifkey="21"
/label=Tet\repressor
/note="E. coli Tn10 tetR repressor Accession X00694"
3'UTR 1782..1921
/vntifkey="50"
/label=Ald\3'\utr
/note="Aldolase 3' untranslated region. Accession x52586"
5'UTR 1933..2042
/vntifkey="52"
/label=Act\SAS
/note="Actin splicing acceptor site. Accession m20310"
CDS 2053..3089
/vntifkey="4"
/label=Hygro\r
/note="Hygromicin B phosphotransferase Accession k01193"
3'UTR 3112..3389
/vntifkey="50"
/label=Act\3'\utr
/note="Actin 3' untranslated region. Accession m20310"
CDS 4335..5195
/vntifkey="4"
/label=Amp\r
/note="B-lactamase gene"
promoter 942..961
/vntifkey="30"
/label=T7\promoter\(10%)
BASE COUNT 1491 a 1549 c 1606 g 1544 t
ORIGIN
1 ctgaccgtat catgatgact ttctccatca tcccatcccc caaggtgtcc gacactgtcg
61 tcgagccgta caatacgact ctctccgtgc accaacttgt ggaaaactcc gatgagtcga
121 tgtgcattga caacgaggca ctgtacgata tttgcttccg caccctgaaa ctgacaacac
181 caacgttcgg tgacctgaac cacttggtgt ctgctgttgt gtccggcgtc acctgctgcc
241 tgcgcttccc tggtcagttg aactctgacc tccgtaagtt ggctgtgaac cttgtcccat
301 tcccgcgtct gcacttcttc atgatgggct tcgccccgct gaccagccgc ggctcgcagc
361 agtaccgcgg tctctccgtg cccgagctaa cgcagcagat gttcgatgcg aaaaacatga
421 tgcaagctgc agatcctcgt cacggccgct acctgacagc gtctgcactc ttccgcggcc
481 gcatgtcgac gaaggaggtt gatgagcaga tgctgaacgt gcagaacaag aactcgtcct
541 acttcattga gtggatcgat cccgaacaac atcaagtcct ctgtttgcga tatcccaccc
601 aagggactca agatggctgt caccttcatt ggcaacaaca cctgcatcca ggagatgttc
661 cgccgtgtgg gagagcagtt caccctcatg ttccgtcgca aggcgttctt gcactggtac
721 actggcgagg gtatggacga gatggaattc acggaggcag agtccaacat gaacgatctc
781 gtgtctgagt accagcagta ccaggatgcc acgattgagg aggagggcga gttcgacgag
841 gaggagcaat actagacgcg gacggggcat ttcccgttcg tcattagcag taggtaatga
901 agatgctcga gggtgctcaa gctgtgtagc gcacgcgccg gtaatacgtc tcactatagg
961 gagatctacc ggcgcgtttc cttacatatt tctctaacag gcacggaagc ctaacaaata
1021 cacttggctt atttttttgc cccctcatgt cttgtacaaa tatttgcgat agcttagcta
1081 tcagccacat taatcaaaca agtataccaa caagcccgaa aacataaact caactgcaac
1141 gaagctttca tatgtctaga ttagataaaa gtaaagtgat taacagcgca ttagagctgc
1201 ttaatgacgt cggaatcgaa ggtttaacaa cccgtaaact cgcccagaga ctaggtgtag
1261 agcagcctac attgtattgg catgtaaaaa ataagcgggc tttgctcgac gccttagcca
1321 ttgagatgtt agataggcac catactcact tttgcccttt agaaggggaa agctggcaag
1381 attttttacg taataaggct aaaagtttta gatgtgcttt actaagtcat cgcgatggag
1441 caaaagtaca tttaggtaca cggcctacag aaaaacagta tgaaactctc gaaaatcaat
1501 tagccttttt atgccaacaa ggtttttcac tagagaatgc attatatgca ctcagcgctg
1561 tggggcattt tactttaggt tgcgtattgg aagatcaaga gcatcaagtc gctaaagaag
1621 aaagggaaac acctactact gatagtatgc cgccattatt acgacaagct atcgaattat
1681 ttgatcacca aggtgcagag ccagccttct tattcggcct tgaattgatc atatgcggat
1741 tagaaaaaca acttaaatgt gaaagtgggt cttaaggatc ctgcccattt agttggcttt
1801 tcccttgtct cgtgtctttt ccgtggaaag gttcccggag taatctgatg gcacagcagg
1861 gaggtgcgcc tgcaggttgg ttaggaaggg gggatgatgt aaaagaagaa aatgggggga
1921 ttcgagcccg ggcacagcaa ggtcttctga aattcatgtt tttttttttt ttactctgca
1981 ttgcagtctc cgctcttatt tagttttgct ttacgtaagg tctcgttgct gccataaaat
2041 aagctactag tgatgaaaaa gcctgaactc accgcgacgt ctgtcgagaa gtttctgatc
2101 gaaaagttcg acagcgtctc cgacctgatg cagctctcgg agggcgaaga atctcgtgct
2161 ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg taaatagctg cgccgatggt
2221 ttctacaaag atcgttatgt ttatcggcac tttgcatcgg ccgcgctccc gattccggaa
2281 gtgcttgaca ttggggaatt cagcgagagc ctgacctatt gcatctcccg ccgtgcacag
2341 ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg ctgttctgca gccggtcgcg
2401 gaggccatgg atgcgatcgc tgcggccgat cttagccaga cgagcgggtt cggcccattc
2461 ggaccgcaag gaatcggtca atacactaca tggcgtgatt tcatatgcgc gattgctgat
2521 ccccatgtgt atcactggca aactgtgatg gacgacaccg tcagtgcgtc cgtcgcgcag
2581 gctctcgatg agctgatgct ttgggccgag gactgccccg aagtccggca cctcgtgcac
2641 gcggatttcg gctccaacaa tgtcctgacg gacaatggcc gcataacagc ggtcattgac
2701 tggagcgagg cgatgttcgg ggattcccaa tacgaggtcg ccaacatctt cttctggagg
2761 ccgtggttgg cttgtatgga gcagcagacg cgctacttcg agcggaggca tccggagctt
2821 gcaggatcgc cgcggctccg ggcgtatatg ctccgcattg gtcttgacca actctatcag
2881 agcttggttg acggcaattt cgatgatgca gcttgggcgc agggtcgatg cgacgcaatc
2941 gtccgatccg gagccgggac tgtcgggcgt acacaaatcg cccgcagaag cgcggccgtc
3001 tggaccgatg gctgtgtaga agtactcgcc gatagtggaa accgacgccc cagcactcgt
3061 ccgagggcaa aggaatagag tagatgccga ccgggatcga tcccccgatc ctaacaccgg
3121 gttgtgtggc caaaattgtt ctgtagtcgc tgtgagttga cacggctagt gcttatgatt
3181 ttcctcgcgt gtggtgcctg tactcagccc tatgccttat ttgcaacaca tttacgtaca
3241 gcgcacaaga gaagagaaga tcacttgaag ataataaata tagggttgta ggcatcttgt
3301 ttaactcaaa ttttctcgtc ttggtgtgtc gacatgattg aaatagtgcc accagttgtg
3361 tttgatgcgt ttgttatcta tgcagtattc tgcaaggcct tgcaggcatg caagctagct
3421 tgtattctat agtgtcacct aaatcgtatg tgtatgatac ataaggttat gtattaattg
3481 tagccgcgtt ctaacgacaa tatgtacaag cctaattgtg tagcatctgg cttactgaag
3541 cagaccctat catctctctc gtaaactgcc gtcagagtcg gtttggttgg acgaaccttc
3601 tgagtttctg gtaacgccgt tccgcacccc ggaaatggtc agcgaaccaa tcagcagggt
3661 catcgctagc cagatcctct acgccggacg catcgtggcc ggcatcaccg gcgccacagg
3721 tgcggttgct ggcgcctata tcgccgacat caccgatggg gaagatcggg ctcgccactt
3781 cgggctcatg agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg ccgggggact
3841 gttgggcgcc atctccttgc accattcctt gcggcggcgg tgctcaacgg cctcaaccta
3901 ctactgggct gcttcctaat gcaggagtcg cataagggag agcgtcgata tggtgcactc
3961 tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg
4021 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
4081 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa
4141 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
4201 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
4261 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
4321 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
4381 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
4441 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
4501 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
4561 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
4621 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
4681 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
4741 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
4801 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
4861 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
4921 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
4981 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
5041 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
5101 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
5161 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
5221 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
5281 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
5341 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
5401 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
5461 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
5521 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
5581 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
5641 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
5701 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
5761 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
5821 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
5881 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
5941 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
6001 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
6061 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
6121 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
6181 attaatgcag
//