LOCUS pTSO-Hyg4 5375 bp DNA circular 19-AUG-2000
SOURCE
ORGANISM
COMMENT This file is created by Vector NTI
http://www.informaxinc.com/
COMMENT VNTDATE|177336533|
COMMENT VNTDBDATE|203522753|
COMMENT VNTNAME|pTSO-Hyg4|
COMMENT VNTAUTHORNAME|Tristan|
COMMENT VNTAUTHOREML|simpson@hhmi.ucla.edu|
FEATURES Location/Qualifiers
CDS 828..1790
/vntifkey="4"
/label=HygR
splicing_signal 791..792
/vntifkey="38"
/label=3'\SAS\site
rep_origin complement(4904..5279)
/vntifkey="33"
/label=ORI
/note="E. coli origin of replication"
CDS 3479..4339
/vntifkey="4"
/label=AmpR
splicing_signal 556..641
/vntifkey="38"
/label=PARP\SAS
misc_feature 1992..2436
/vntifkey="21"
/label=Tb\minicircle\sequence
promoter 281..550
/vntifkey="29"
/label=PARP\promoter
/note="Not sure of limits to sequence"
promoter 5350..5369
/vntifkey="30"
/label=T7\promoter
BASE COUNT 1370 a 1292 c 1223 g 1490 t
ORIGIN
1 gaattcgagc tcggtacccg gcctaaatgc cgctgattca ccacaaagtg cttttcagtg
61 cccccaaact ggattgccaa acttctgaca agtgtattgt tccgagtatg gtgagcgaat
121 cccttctcta ttatcaattc tcgaaaactc ttcgggagtc agggcccacg gaaccgaggg
181 ggcggatgag ggagaggggg aaacaacaat tccgcaccgc gaacaggcac cgctcccctg
241 gacctctccc tcagatgtga ggtgcagtca gcctttgttg tcattggggt taagcggaaa
301 ggtgtgtgtc agtaggttgt gaggtgaaag cgttttcaga tgcatagtga gcttaatgtc
361 cttttcacag tatatcgtgt ctgataggta tctcttatta gtatagtcga atactagtca
421 atagtgcgtt ttgtgcaaaa tgtccatttt gtggcagtga tggggttgtt ttatgctatt
481 ccgtgtctct gggtgggcgt gcattgaaaa taggggttat cgggtgagta ctgagtttaa
541 catgttctcg tgatcgctgc acgcgccttc gagttttttt tccttttccc catttttttc
601 aacttgaaga cttcaattac accaaaaagt aaaattcaca agctcccctc gaggtcgacg
661 gtatcgataa gcttgatatc gaattcctgc agcccggggg atccactagt tctactgagt
721 ttagatgttc tcgtgatcgc tgcacgcgcc ttcgagtttt ttttcctttt acccattttt
781 ttcaacttga agacttcaac ggaattcctc ccggggggca atgagatatg aaaaagcctg
841 aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc
901 tgatgcagct ctcggagggc gaagaatctc gtgctttcag cttcgatgta ggagggcgtg
961 gatatgtcct gcgggtaaat agctgcgccg atggtttcta caaagatcgt tatgtttatc
1021 ggcactttgc atcggccgcg ctcccgattc cggaagtgct tgacattggg gaattcagcg
1081 agagcctgac ctattgcatc tcccgccgtg cacagggtgt cacgttgcaa gacctgcctg
1141 aaaccgaact gcccgctgtt ctgcagccgg tcgcggaggc catggatgcg atcgctgcgg
1201 ccgatcttag ccagacgagc gggttcggcc cattcggacc gcaaggaatc ggtcaataca
1261 ctacatggcg tgatttcata tgcgcgattg ctgatcccca tgtgtatcac tggcaaactg
1321 tgatggacga caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg atgctttggg
1381 ccgaggactg ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc aacaatgtcc
1441 tgacggacaa tggccgcata acagcggtca ttgactggag cgaggcgatg ttcggggatt
1501 cccaatacga ggtcgccaac atcttcttct ggaggccgtg gttggcttgt atggagcagc
1561 agacgcgcta cttcgagcgg aggcatccgg agcttgcagg atcgccgcgg ctccgggcgt
1621 atatgctccg cattggtctt gaccaactct atcagagctt ggttgacggc aatttcgatg
1681 atgcagcttg ggcgcagggt cgatgcgacg caatcgtccg atccggagcc gggactgtcg
1741 tcgccgatag tggaaaccga cgccccagca ctcgtccgag ggcaaaggaa tagagtagat
1801 gccgaccggg atcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga
1861 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccctaga
1921 cgcagatatg aagcagtatt tatcacatta atcccactct accaccgaat aatcccatta
1981 atttcatcct ccataaataa ataaaataat aaatagattt taataataat atcatttaat
2041 tataatatat catcactcac tatagaaaat ggcagttttg ttgatcttat atctatatat
2101 atcattataa tactacaata catatctatt atctcatttc tttcccacta ggtaatcccg
2161 agaatgagaa taaattaata aatagtaata taagaaataa atataaaatc atcaccgcga
2221 gtttcgaggt attataacct cttcagttta attataattg ctatatatct aacttatata
2281 tttatctatt actatatttt attccttaag tagactataa aaacacaata ttaattacac
2341 tagatatcac cccaaaaccc tgaaatctcg aggaaaaacc atgtgtgtat tacaccaacc
2401 tatttttgca cgcccataag attttgggtt ttttcaagcc atttttcttc gatttttacc
2461 cgattttcca taattttctt acatccacta ctaatctctt tcaattaata actctgtgct
2521 tcacttctga ttccctccat tatctcacca ctccaatcat aaataaaata atagatacag
2581 tataaatata aatctcattt tacaggtata aatcacagtc tctcagattc tatttcactt
2641 ttattggatt tatataattc ttcactttat tatctattca ttgttctatc tattaatatc
2701 ttctctttcc cttagtaata aaataaaata atagatatat taacagtaat taataattat
2761 atatattaca tcgtctcttc tactgcttta tcagtttagt tagtcattat atatatcata
2821 tttaacttaa tatattacta ctacttatct attaaattat tttacctttc tagcattcta
2881 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcgat cctagagctt
2941 ggcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
3001 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
3061 ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc
3121 tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg
3181 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
3241 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg
3301 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
3361 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
3421 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
3481 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
3541 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
3601 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
3661 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
3721 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
3781 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
3841 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
3901 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
3961 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
4021 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
4081 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
4141 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
4201 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
4261 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
4321 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
4381 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
4441 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
4501 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
4561 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
4621 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
4681 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
4741 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
4801 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
4861 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
4921 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
4981 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
5041 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
5101 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
5161 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
5221 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
5281 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggct
5341 tatcgaaatt aatacgactc actataggga gaccg
//