Lab Vectors

List of vectors:

pT7KO1c - for replacing the first allelle of glutamate dehydrogenase of T. brucei with T7 RNA polymerase. Click here (shift-click if using Netscape on a PC, Ctl-click if using Netscape on a Mac)) to download a file with the features that can be viewed with the NTI viewer. Click here to download the NTI Viewer for any platform. Click here to see this sequence in Genbank text format.

pEKA2 revised - for replacing the first allelle of HGPRT in L. tarentolae with T7 RNA polymerase. Click here to download a file to view with the NTI Viewer. Click here to see a text file in Genbank Format.

pHD360 (from C. Clayton). Click here to download a file to view with the NTI Viewer. Click here to see a text file in GenBank format.

pLEW82 (Wirtz and Cross)

File in Genbank format.

pLEW90 (Wirtz and Cross). Click here to see a text file in GenBank format.

pLEW100 (Wirtz and Cross). Click here to see a text file in GenBank format.

pLEW79 corrected (Wirtz and Cross). Click here to see a text file in GenBank format.

pX (Beverley). Click here to see the file in Genbank format.

8. pTS0-Hyg (C.C. Wang). Click here to see the file in Genbank format.

 pZJM plasmid for RNAi in T. brucei (Englund lab). Click here to see the file in Genbank format.

pTUTi-H2H. This is the construct we made for cloning Tb TUTase fragments in a head to head configuration for RNAi. Click here to see sequence in Genbank format.

pSAP1. This is a new dual epitope TAP vector with the strepavidin binding peptide (Wilson et al, 2001) substituted for the calmodulin binding peptide. Click here for the sequence in Genbank format. If you have the free NTI viewer, it will open in graphic form. The plasmid has the L. major RET2 gene in the expression site. Release this fragment with BamHI and XbaI and clone your gene of interest in frame with the C-terminal tags. Select in E. coli with 50 ug/ml amp.

pXLtREL1-mod. This is the original TAP vector with the calmodulin binding peptide and the protein A peptide (Aphasizhev et al., 2002). Remove the insert with XbaI and BamHI and clone in your gene. Click here to see the sequence in Genbank format. If you have the free NTI viewer, it will open in graphic form. Otherwise, download and open in a text editor such as Notepad.

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Maintained by Larry Simpson