LOCUS KTKPMCC 1058 bp DNA circular ORG 16-NOV-2002
DEFINITION T.brucei kinetoplastid minicircle H39 DNA, complete.
ACCESSION M15323 M15302
KEYWORDS minicircle.
SOURCE
ORGANISM Trypanosoma brucei
Eukaryota; Euglenozoa; Kinetoplastida; Trypanosomatidae; Trypanosoma.
COMMENT A 13 base pair sequence, within the conserved region of about 130
base pairs (positions 501-633), is conserved among kinetoplastid
genera [1]. Minicircle fragments containing the conserved sequence
and a 5' adjacent segment, starting at position 199 with an 18 base
pair sequence that occurs as 3 or 4 pairs of imperfect inverted
repeats in African trypanosomes [2], exhibit anomalous
electrophoretic mobility in polyacrylomide gels [1]. The position
of the oligo (dA) tracts in the fragments appears to influence this
anomalous ability [1]. The presence of conserved features
independent of conserved nucleotide sequence and the lack of
conserved open reading frames among these minicircles suggests that
mincircles may have a function other than encoding protein [1]. The
18 base pair sequence is polar, and one half is almost perfectly
conserved [2]. The distribution of the 18 base pair sequences in
minicircles defines two classes of sequences: A segments vary
considerably in length and contain about 10% more "g" + "c" than B
segments, which are all about 100 base pairs long. B segments along
with their terminal 18 base pair sequences superficially resemble
insertion sequences [2]. Five 14 base pair long degenerate tandem
repeats are located at positions 301-432.
COMMENT EMBLRFLN|RN [1]
COMMENT EMBLRFLN|RP 1-1058
COMMENT EMBLRFLN|RX MEDLINE; 86174895.
COMMENT EMBLRFLN|RA Jasmer D.P., Stuart K.D.;
COMMENT EMBLRFLN|RT "Conservation of kinetoplastid minicircle characteristics without
COMMENT EMBLRFLN|RT nucleotide sequence conservation";
COMMENT EMBLRFLN|RL Mol. Biochem. Parasitol. 18:257-269(1986).
COMMENT EMBLRFLN|RN [2]
COMMENT EMBLRFLN|RC 18 bp inverted repeats and flanks
COMMENT EMBLRFLN|RX MEDLINE; 86174900.
COMMENT EMBLRFLN|RA Jasmer D.P., Stuart K.;
COMMENT EMBLRFLN|RT "Sequence organization in African trypanosome minicirclesw is defined by 18
COMMENT EMBLRFLN|RT base pair inverted repeats";
COMMENT EMBLRFLN|RL Mol. Biochem. Parasitol. 18:321-331(1986).
COMMENT This file is created by Vector NTI
http://www.informaxinc.com/
COMMENT ORIGDB|EMBL
COMMENT VNTDBDATE|283442086|
COMMENT VNTNAME|KTKPMCC|
COMMENT VNTAUTHORNAME|Larry Simpson|
COMMENT VNTOAUTHORNAME|UNKNOWN|
FEATURES Location/Qualifiers
source 540..539
/db_xref="taxon:5691"
/kinetoplast
/organism="Trypanosoma brucei"
/vntifkey="98"
misc_feature 1..9
/vntifkey="21"
/label=CSB-1
/note="Add by Min Hong"
misc_feature 28..35
/vntifkey="21"
/label=CSB-2
/note="Add by Min Hong"
misc_feature 75..87
/vntifkey="21"
/label=CSB-3
/note="Add by Min Hong"
misc_feature 187..214
/vntifkey="21"
/label=gMURF2(938-963)
/note="Add by Min Hong"
misc_feature 377..404
/vntifkey="21"
/label=gMURF2(936-963)
/note="Add by min hong"
misc_feature 860..905
/vntifkey="21"
/label=gMURF2(977-1015)
/note="Add by min hong"
repeat_region complement(503..520)
/vntifkey="34"
/label=RP1
/note="Add by Min Hong"
repeat_region 607..624
/vntifkey="34"
/label=RPa
/note="Add by Min Hong"
repeat_region complement(738..754)
/vntifkey="34"
/label=RP2
/note="Add by Min Hong"
repeat_region 847..864
/vntifkey="34"
/label=RPb
/note="Add by Min Hong"
misc_feature 1018..90
/vntifkey="21"
/label=CON
misc_feature 661..688
/vntifkey="21"
/label=gCR4(129-152)
BASE COUNT 384 a 75 c 225 g 374 t
ORIGIN
1 agggcgtgca aaaatacaca tacacaaatc cgtgctattt tggggcattt tttgaggtcc
61 gaggtacttc gaaaggggtt ggtgtaatac acacacggtt tttcaaggga tttctcggtt
121 tatctgggtt ttatgggttt tcggggtctg gaggtttcaa aataaaaaga aaatatatta
181 ataaatagaa gtacgtcata atatatataa ttaatatact gtaaagaggg aatatacacc
241 ctctatataa taagatacct atataattgt aataagtata attatctatc tattatttat
301 ttttattgtt tggagcgggt aattaggtgt ttgatggttg atttaggtgt ttattgtaac
361 tgcgaatctg actaataaag ataagataat agataggagt ataaatgata attgagtgat
421 gagatatata attaaattta tgaggatttt attgtagatg atagtgttat atgatgtaat
481 atatattaat tattttgtat attatttatt attttatttt gttcttggag gtgatgatga
541 agctttgatc tactgttgta attgctcata tctaacttct gggattaatt gaatgcctaa
601 aagagagaag tataataata gatacgagat aaataaatat atacatagaa attaatacat
661 aaatcacaaa cgaataaggg gcagaagagt tgtatgatat attaaatatg atgaataata
721 atgattaaat taatacttat ttattatatt attttaatta gtggggttgt taggaggatt
781 aatttgatgg gataaatggg gtggtaagag tggtaagact gaaaaagtta atggtaattg
841 gtcgtaaaat atggttatag aataaattaa agtgtaaggg tgatgtgaag aaagcgttat
901 ggagatttaa tattggagat ttaatattgg agatttaata ttggagattt aatattggag
961 aaatttaata ttggaggctg tagtaggtag gctatgagtg ttgaacgaaa tcatgaaaaa
1021 aatcggggaa aattgcctcg aaaaaaccga aaatctta
//