Frameshift editing of cytochrome oxidase subunit III - L. tarentolae

       1  AUGGCUUUUA UAUUAUCAUU UUGAAUGAUA UUUUUAUUAG AUUCUGUUAU
 
      51  UGUUUUGUUA UCUUUUGUAU GUUUUGUAUG UGUGUGAAUA UGUGCAUUGU
 
     101  UGUUUUCAAC UGUAUUAUUA GUGUCAAAAU UAAAUAAUAU AUAUUGUACU
 
     151  UGAGACUUCA CUGCUUCUAA GUUCAUUGAU GUUUACUGAU UUACUAUUGG
 
     201  UGGUAUGUUU UCAUUAGGAC UUUUAUUACG UUUAUGUUUA CUUUUAUAUU
 
     251  UUGGGCAUUU AAAUUUUGUU AGUUUUGAUU UGUGUAAAGU AGUGGGAUUU
 
     301  CAAUGAUACU GAGUUUAUUU UAUUUUUGGA GAAACUACAA UAUUUAGUAA
 
     351  UUUAAUUUUA GAAAGUGAUU AUAUGAUAGG AGACUUACGU UUAUUACAAU
 
     401  GUAAUCAUGU AUUAACUUUA UUAAGUUUAG UUAUAUAUAA AUUAUGAUUA
                                                         
     451  UCAGCUGUCG AUGUAAUACA UUCAUUUGCA AUAUCAAGUU UAGGAGUUAA

 3'-[AU]U AUAUCUaaUaUaUGGAUAUAUU 
           :||||||:|||||||:          
     501  AGUAGAuuGuAuACCUGGUC GCUGUAAUGA AAUAGUUCUA UUUUCAUCAA
           V  D  C  I  P  G  R   C  N  ..
 
     551  AUAAUGCCAC AGUAUAUGGA CAAUGUAGUG AACUCUGUGG UGUCUUACAU
 
     601  GGAUUUAUGC CAAUAGUAAU UUGUU   

Note that the gRNA is in cis.